Light-Controlled Microecological Hydrogel For Diabetic Alveolar Bone Defect Reconstruction

The alveolar bone defect has led to approximately one billion missing teeth in China. Among these patients, about 13% are also affected by diabetes mellitus. Diabetic alveolar bone is characterized by “hypoxia-inflammation inhibitory osteogenesis”,. To address these issues, this project aims to develop a light-controlled “Chlorella-BMSC symbiotic microecological hydrogel” for diabetic alveolar bone defect reconstruction. The study demonstrates that chlorella creates a favorable environment for bone regeneration and BMSC growth through photosynthesis, including long-term and precise release of oxygen, and scavenging of ROS. Furthermore, BMSC protects chlorella through the paracrine modulation of macrophage polarization and promotes osteogenic differentiation. On this basis, this project developed a light-controlled “Chlorella-BMSC symbiotic microecological hydrogel” based on the symbiotic relation, optimized its preparation parameters and performance, and investigated its oxygen release, immuno-inflammation regulation, and osteogenesis induction capabilities using an in-vitro diabetic inflammation model and an in-vivo diabetic alveolar bone defects model. The project also elucidated the regulatory mechanisms of the “oxygen release-anti-inflammation-osteogenesis” pathway. The present study has initially revealed a mutually beneficial symbiotic relationship between chlorella and bone marrow mesenchymal stem cell (BMSC) in an in-vitro diabetes model. A light-controlled “Chlorella-BMSC symbiotic microecological hydrogel” based on the symbiotic relationship between chlorella and BMSC is successfully produced. The microecological hydrogel showed controlled oxygen release, promoted anti-inflammation ability and enhanced osteogenic activity. This study will provide a theoretical basis and material foundation for the clinical treatment of diabetic alveolar bone defects.