Study On Mechanism Of Rasal1 Regulating Progression Of Oral Leukoplakia

To investigate the mechanism and regulation of RASAL1 on the progression of oral leukoplakia and to find its therapeutic target. DOK culture system was established, control group and experimental group were set up. The expression of RAS-GTP was detected by ELISA. The mRNA expression of RASAL1 was detected by PCR. The expression levels of RASAL1 and pathway-related proteins were detected by WB. At the same time, RASAL1 overexpression and knock-down system were established to verify the regulatory effect of RASAL1 on the progression of oral leukuplakia. The mRNA and protein expression of RASAL1 were significantly increased (P < 0.001), and the activity and expression of RAS-GTP were significantly decreased (P < 0.01), which realized the pathway inhibition process. The increase and decrease of RASAL1 expression can regulate the development of oral leukoplakia.