Tdn-Modified Coating For Titanium Bioaffinity And Osseointegration

Titanium (Ti) is widely used in medicine due to its excellent biomechanical properties, but precise nano-fabrication of its surfaces is challenging. We developed a DNA-nanocoating system to functionalize Ti surfaces and explored the effects of tetrahedral DNA (TDNs) on rat bone marrow mesenchymal stem cells (rBMSCs) adhesion, proliferation, and osteogenic differentiation, as well as their biological effects under hypoxic and inflammatory conditions. Clickable Ti surface made via hydroxylation, silanization, click chemistry. FE-SEM, XPS analyzed roughness, composition. Confocal imaging showed 30 nm and 7 nm-TDNs on Ti. Contact angle tested hydrophilicity. rBMSCs cultured on TDNs-modified Ti for adhesion, proliferation (CCK-8), osteogenic differentiation (ALP, ARS, qRT-PCR). In vivo, TDNs-modified Ti rods implanted in mice femurs, bone formation analyzed by micro-CT, H&E, Van Gieson staining. 1. Developed a DNA nanocoating system for titanium surfaces using hydroxylation, silanization, click chemistry, and base-pairing. 2. Assembled 30 nm and 7 nm TDNs; 30 nm TDNs showed better cellular responses and osteointegration via focal adhesion pathway. 3. 30 nm TDN-modified surfaces performed better under inflammatory and hypoxic conditions. We functionalized Ti surfaces with ssDNA overhangs via click reaction and assembled 30 nm and 7 nm TDNs. The 30 nm TDNs significantly enhanced Ti surface hydrophilicity, cell proliferation, and osteogenic differentiation, and maintained performance under inflammatory and hypoxic conditions.