Single-Cell Immune Characteristics Of Periodontitis And Pss

Conduct a comprehensive and high-resolution analysis and comparison of the immune cell blueprints in periodontitis (PD) and primary Sjögren's syndrome (pSS), to reveal the similarities and differences between the two diseases at the single-cell level. Integrate the single-cell transcriptomic data of peripheral blood mononuclear cells (PBMCs) from healthy controls (HC), PD patients, and pSS patients. Use Seurat for quality control, deduplication, normalization, and dimensionality reduction. Perform t-SNE and k-means clustering to analyze cell subpopulations, including T/NK cells, myeloid cells, and B cells. Additionally, use NicheNet to analyze the interactions between myeloid cells and CD8 Tn cells. Identify nine T/NK cells subclusters, seven myeloid cells subclusters, and five B cells subclusters. The proportion of CD8 naive T (Tn) cells was significantly downregulated in both PD and pSS. HLA-DRA mono cells showed a significant decrease in PD and a trend of decrease in pSS. Additionally, immature B cells were upregulated in PD but showed an opposite trend in pSS. The intracellular communication analysis between myeloid cells and CD8 Tn cells suggested that myeloid cells may promote the exhaustion of CD8 Tn cells. In summary, this study reveals the association between PD and pSS at the single-cell level, providing new insights into the common molecular mechanisms underlying these two diseases.