Immune Reprogramming Of Photothermal Engineered Arginine Bacteria In Stomatology Disease

Activating the immune system plays an important role in fighting against metastatic tumor and preventing postoperative recurrence. In this project, indocyanine green was connected to L-arginine engineering bacteria——ICG-L(EcN) to prepare photothermal arginine engineering bacteria, and to study its role in immune reprogramming mechanism. Firstly, the amount of ICG on L(EcN) was observed, and ICG-L(EcN) with higher connection amount was prepared. 3D cell spheres penetration experiment were used to detect the tumor targeting effect of photothermal engineered arginine bacteria. Then, the changes of immune microenvironment was observed. The killing of tumor by ICG-L(EcN) under the action of immune cells, and the ICD induced by ICG-L(EcN) were observed to explore its role in reprogramming immune cells and the mechanism of its regulation. Moreover, ICG-L(EcN) was proved to be effective in treating oral squamous cell carcinoma in vivo, and the mechanism of ICG-L(EcN) reprogramming immune cells was preliminarily investigated. ICG-L(EcN) with better photothermal effect and arginine production performance was obtained. ICG-L(EcN) has low cytotoxicity and high biocompatibility, which can effectively target tumor cells and penetrate into the tumor sphere for photothermal treatment. At the same time, ICG-L(EcN) effectively induced immunogenic cell death and promoted immunotherapy of oral squamous cell carcinoma. Photothermal L-arginine bacteria can effectively target and treat oral squamous cell carcinoma through immunotherapy, and provide a new idea for the prevention and treatment of oral squamous cell carcinoma.