Click Chemistry-Driven Hydrogel For Multistage Management Of Infected Wound

This study aims to develop a click chemistry-based multifunctional hydrogel for comprehensive and effective multistage management of infected wound healing. An HKT hydrogel hybrid system, comprising methacrylated hyaluronic acid, sulfhydryl kappa-carrageenan, and tannic acid, was developed using click chemistry for precise polyphenol integration.The mechanical properties, rheological behavior, tissue adhesiveness, antioxidant activity, antibacterial efficacy, and biocompatibility of the hydrogel are systematically evaluated. Additionally, its ability to modulate inflammation and promote angiogenesis is assessed. In vivo studies are conducted to determine the hydrogel's therapeutic efficacy in infected wound healing. The composite hydrogel possesses suitable mechanical properties, favorable rheology, and strong tissue adhesiveness. It exhibits excellent antioxidant (DPPH scavenging >90%) and antibacterial (efficacy >99%) activities, along with outstanding biocompatibility, effectively regulating inflammation and promoting angiogenesis.Furthermore, In vivo studies demonstrate a 48.4% improvement in wound healing efficiency compared to the control group, with immunohistochemical analysis conducted to elucidate the underlying mechanisms by which HKT hydrogel enhances wound healing. This click chemistry-based hydrogel exhibits excellent antioxidant, antibacterial, anti-inflammatory, and angiogenic properties, making it a promising candidate for infected wound healing through multistage comprehensive management.