Microfluidic Preparation Of Cerium-Cmc Microspheres For Oral Tissue Repair

Due to the complex environment of the oral cavity, traditional drug delivery methods result in rapid drug loss, and the treatment of periodontitis, post-extraction wounds, and oral mucosa-related diseases still fail to meet clinical demands, and is of clinical significant to design a novel material for treating oral-related diseases and tissue repair. In this study, microfluidic technology was employed to prepare microspheres using carboxymethyl cellulose as the base material.Variable valence cerium ions was used as the crosslinking agent.Magnesium-containing bioactive glass was doped into the microspheres as the functional component. The influence of microfluidic parameters on microsphere size and morphology was first investigated. Carboxymethyl cellulose microsphere scaffolds containing different bioactive glass microspheres were then obtained through freeze-drying, and their composition, structure, and morphology were characterized. Subsequently, the microspheres were tested for cell culture, and their cytocompatibility was evaluated. When the flow rate ratio was 15:1 and the carboxymethyl cellulose content was 1%, uniform spherical droplets approximately 300 micrometers in size were obtained. Under this preparation condition, the incorporation of varying amounts of bioactive glass microspheres generated corresponding droplets, which exhibited surface wrinkles after freeze-drying, revealing the presence of the embedded microspheres. Cytocompatibility evaluation confirmed that the prepared microspheres were non-toxic and exhibited cytocompatibility. The carboxymethyl cellulose microspheres prepared by microfluidic technology have a regular spherical morphology and good biocompatibility, demonstrating promising application prospects in oral medicine.