Study On Electrospinning Artificial Periosteum To Promoting Osteogenesis

A nanocomposite artificial periosteum loaded with black phosphorus and vascular endothelial growth factor was fabricated using electrospinning technology. The physicochemical characteristics, biocompatibility, in vitro and in vivo osteogenic capabilities of this material were systematically investigated. The electrospinning solution was prepared by dissolving L-polylactic acid particles in methylene chloride. Black phosphorus/vascular endothelial growth factor nanocomposite artificial periosteum (PLLA, PLLA/BP, PLLA/VEGF, PLLA/BP/VEGF fiber membranes) was fabricated using electrospinning technology. The physical and chemical properties were studied in vitro, their ability to promote osteogenic differentiation was investigated in vivo through SD rat skull defect repair experiments. Animal experiments have been approved by the Institutional Animal Ethics Committee. (1) Characterization: All the membranes exhibited excellent mechanical properties, and the incorporation of black phosphorus and growth factors enhanced the hydrophilicity of the membranes. (2) Biocompatibility: Live/dead fluorescence staining and CCK-8 results indicated that the fiber membrane exhibited superior biocompatibility. (3) In vitro osteogenic differentiation performance: ALP, ARS staining, and qRT-PCR further confirmed that the fiber membrane markedly promoted the osteogenic differentiation of BMSCs. (4) Establishment of animal model and composite membrane implantation: Micro-CT revealed increased new bone in the defect area compared to the control group, while the composite membrane effectively promoted the high expression of osteogenesis-related genes. Electrospun artificial periosteum loaded with black phosphorus and vascular endothelial growth factor has better osteogenic mineralization promoting performance, which can provide certain research basis for the progress of periosteum tissue engineering.