A Microenvironment Responsive Nanoparticle Regulating Osteoclast Fate To Alleviate Periodontitis

We explored a shell-core structured biomaterial coated with a metal phenolic network, which exhibited adaptive sensitivity to pH values, to conduct bone repair in periodontitis. Materials: Tannic acid, Cerium sulfate, NaOH, h gelatin nanoparticle powder, BSA, RhodaMine labeled BSA (BSA/RBITC), Murine sRank-Ligand (Rankl), Recombinant Macrophage Colony Stimulating Factor 1 (M-CSF), Lipopolysaccharide (LPS), Recombinant Mouse BMP9, phosphate buffered saline (PBS). Method: The animal experiments were approved by the Animal Ethics Committee of Chongqing Medical University. SD rats were assigned to four groups to study the effects of periodontitis repair as group 1 (control), group 2 (periodontitis), group 3 (4-week treatment), group 4 (12-week treatment) randomly (n≥3) . Extracellularly, it rapidly responded to lower pH, achieving specific release in an inflammatory microenvironment. Intracellularly, it impacted the formation, function, and differentiation of osteoclasts through the macrophage-osteoclast axis, thereby promoting bone defect repair. In vivo and in vitro studies showed the biomaterial regulated osteoclasts to optimize periodontitis treatment strategies, highlighting the potential of modified nanobiomaterials for clinical application.