The Influence Of MYDGF On The Osteogenic Differentiation Of DPSCs

Bone marrow-derived growth factors, as positive regulators of bone homeostasis, can participate in the regulation of bone metabolism. To explore whether MYDGF can promote the differentiation of human dental pulp stem cells towards odontoblastic lineage and promote the root development of young permanent teeth, and to explore the possible mechanisms. Isolate and culture primary human dental pulp stem cells. Detect cell surface markers by flow cytometry and immunofluorescence staining. After mineralization induction, detect the degree of cell mineralization by ALP and alizarin red staining. Use qRT-PCR and WB to detect the expression levels of genes and proteins related to odontoblastic differentiation in each group of cells. Flow cytometry and immunofluorescence assays revealed that hDPSCs highly expressed the surface markers of mesenchymal lineage cells. After osteogenic induction, ALP and alizarin red staining assays indicated that rhMYDGF significantly promoted osteogenesis of hDPSCs. qRT-PCR analysis showed that after rhMYDGF induction and culture, the expressions of osteogenic-related genes BSP, RUNX2, OPN, COI1, DMP1 and DSPP were significantly upregulated. Western blotting assays revealed that the expression levels of osteogenic-related proteins DMP1, RUNX2 and OCN were significantly increased. Under in vitro conditions, MYDGF can promote the odontoblastic differentiation of dental pulp stem cells, providing a new idea for the regenerative endodontic treatment of young permanent teeth. MYDGF is a potential cytokine in dental pulp tissue engineering.