Aggregation-Induced Emission-Based Lateral Flow Immunoassay for Ultra-Sensitive and On-Site Detection of Porcine Epidemic Diarrhea Virus
The porcine epidemic diarrhea virus (PEDV) has inflicted substantial economic losses on the swine industry, underscoring the need for sensitive point-of-care diagnostics. While lateral flow immunoassays (LFIA) offer rapidity and ease of use, traditional labels like colloidal gold suffer from limited sensitivity. Herein, we developed an aggregation-induced emission (AIE)-based LFIA, termed PED-ALFIA, for the highly sensitive detection of the PEDV antigen. PED-ALFIA exhibited a detection limit of 2.44 × 10<sup>2</sup> TCID<sub>50</sub>/mL, which represents a 256-fold improvement in sensitivity over commercial colloidal gold kits and a 4-fold enhancement compared to our previously developed PED-TRFIA. The assay showed no cross-reactivity with other common swine viruses and demonstrated high reproducibility. When tested on clinical samples (n = 42), results showed 100% concordance with qPCR. Utilizing a portable fluorescence reader, the assay can be completed within 10 min, establishing PED-ALFIA as a sensitive, specific, and rapid on-site tool for the early diagnosis of PEDV.
Authors
- Hongwei Shi
- Xufei Feng (ORCID: https://orcid.org/0000-0002-4507-9774)
- Qian He (ORCID: https://orcid.org/0000-0001-5765-496X)
- Bin Wang (ORCID: https://orcid.org/0000-0002-0068-2887)
- Wei Hou (ORCID: https://orcid.org/0000-0003-1441-0708)
- Haidong Wang (ORCID: https://orcid.org/0009-0002-6907-7172)
- Jianjun Geng
Institutions
- Shanxi Agricultural University (CN)
- Jinzhong University (CN)
- Taiyuan University of Technology (CN)
Publication Details
- Journal
- Biosensors
- Published
- 2025-11-03
- DOI
- https://doi.org/10.3390/bios15110736
- Citations
- 1
- Primary Topic
- Animal Virus Infections Studies
- Type
- article
- Field-Weighted Citation Impact
- 1.19