Effects of Voluntary Ingestion of Synthetic Delta-9-Tetrahydrocannabinol on the Hedonic Value of Rewarding and Aversive Substances and CB1 Receptor Expression

Separate, linear, mixed-effect models revealed that THC dose-dependently increased hedonic reactions to both sucrose (0.1 M and 0.5 M) and alcohol (10% and 40% ETOH) compared with control. Comparatively, THC reduced aversion to alcohol (10% and 40%) and quinine compared with vehicle. Western blot data analyzed <i>via</i> one-way Analysis of Variance (ANOVA) followed by Tukey's <i>post hoc</i> analysis revealed that repeated oral THC consumption dose-dependently downregulated cannabinoid receptor 1 receptor expression in the dorsal hippocampus, while no significant changes were observed in the nucleus accumbens or amygdala. Conclusions:These findings suggest that oral THC enhances the hedonic value of rewarding and aversive substances and provide insights into the neurobiological mechanisms that may contribute to the bidirectional use of cannabis and alcohol.