In vitro evaluation of stability and hemostatic efficacy of single-donor lyophilized canine plasma
The lyophilization process did not significantly affect the concentrations or activities of major plasma proteins, including coagulation factors and anticoagulant proteins. Storage at -80 °C and 4 °C for 45 days preserved the stability of biochemical and hemostatic parameters. However, storage at room temperature resulted in minor reductions in select coagulation factors (such as factors II, V, and VIII). PT, aPTT, and thromboelastography parameters remained within the normal reference ranges, confirming the short-term stability of the product. Changes in the reconstitution volume affected plasma concentration and highlighted the potential of lyophilized plasma as a rapid resuscitative product in veterinary medicine.
Authors
- Dong‐In Jung (ORCID: https://orcid.org/0000-0002-5116-6006)
- Kyu‐Woan Cho (ORCID: https://orcid.org/0000-0003-1301-4033)
- Sumin Cha (ORCID: https://orcid.org/0000-0001-9072-4528)
- Hyeona Bae (ORCID: https://orcid.org/0000-0002-2888-5782)
- Changkeun Kang (ORCID: https://orcid.org/0000-0002-5114-5096)
- Do‐Hyeon Yu (ORCID: https://orcid.org/0000-0001-7645-6926)
- Chaewon Shin (ORCID: https://orcid.org/0009-0002-7337-5092)
Institutions
- Gyeongsang National University (KR)
Publication Details
- Journal
- Frontiers in Veterinary Science
- Published
- 2025-11-03
- DOI
- https://doi.org/10.3389/fvets.2025.1663953
- Primary Topic
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Research Foundation
- Ministry of Agriculture, Food and Rural Affairs
- National Research Foundation of Korea
- Ministry of Science and ICT, South Korea
- Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry