Formulation and Characterization of PLGA Minocycline Microneedles for Enhanced Skin Deposition and Antibacterial Activity in Acne Treatment

Acne is a multifactorial skin condition characterized by an infection in the <i>pilosebaceous</i> units in the skin. Patients with acne suffer from comedones, papules, pustules and nodules or cysts in severe cases. These clinical features might cause disfigurmentation, depression, anxiety and significantly impact the quality of life of patients. Systemic and continuous exposure of antibiotics put patients at risk of developing systemic toxicity, bacterial resistance and gut dysbiosis. Microneedles offer an innovative approach of providing targeted topical delivery of minocycline while insuring efficient permeation through skin layers. <b>Methods:</b> minocycline microneedles were formulated using casting method and characterized for insertion ability, mechanical strength, drug content, antibacterial activities, deposition and dissolution behavior using ex vivo full-thickness rat skin. <b>Results:</b> Insertion tests confirmed effective skin penetration and mechanical integrity with only 9.5% height reduction. Drug content was 673.06 ± 5.34 µg/array. Dissolution occurred within 2 min in skin, indicating user-friendly wear time. Ex vivo Franz diffusion studies showed 26% of the drug deposited into the skin, significantly higher (<i>p</i> = 0.0068) than the 18.3% that permeated through it. Antibacterial testing revealed strong activity against <i>S. aureus</i>, <i>S. epidermidis</i>, and <i>C. acnes</i>, with MIC values < 0.146 µg/mL and MBC values ranging from 9.375-18.75 µg/mL. <b>Conclusions:</b> The result of this research demonstrate that minocycline microneedles effectively deliver minocycline into the skin highlighting their potential as a safer and more efficient alternative for acne therapy.