Characterization of a Novel Triterpenoid Saponin from Glinus oppositifolius Aerial Parts with Enzyme Inhibitory Potential

Abstract: A new triterpenoid saponin, namely spergulin C (1), together with four known structures, 3-O-β-Dxylopyranosyl- spergulagenin A (2), vanillin (3), trans-ferulic acid (4), and cinnamic acid (5), were isolated from aerial parts of Glinus oppositifolius. The structures of all compounds were elucidated using comprehensive 1D and 2D NMR techniques, mass spectroscopy, and comparison with the literature. In addition, compounds 3-5 exhibited prominent effects against Xanthine Oxidase (XO) activity, with the IC50 values ranging from 5.37 to 106.69 (μM). In contrast, compounds 1 and 2 showed significant α-glucosidase (α- Glu) inhibitory activity with the IC50 values of 31.23 ± 0.45 (μM) and 13.99 ± 0.33 (μM), respectively. results: A new triterpenoid saponin, namely spergulin C (1), together with four known structures spergulin A (2), vanillin (3), trans-ferulic acid (4), and cinnamic acid (5), were isolated from aerial parts of Glinus oppositifolius. The structures of all compounds were elucidated based on the comprehensive 1D and 2D NMR techniques, mass spectroscopy, and by comparison with the literature. In addition, compounds 3-5 exhibited prominent effects against xanthine oxidase (XO) activity, with the IC50 values ranging from 5.37 to 106.69 (µM). In contrast, compounds 1 and 2 showed significant α-glucosidase (α-Glu) inhibitory activity with the IC50 values of 31.23 ± 0.45 (µM) and 13.99 ± 0.33 (µM), respectively.