Comprehensive evaluation of diverse massively parallel reporter assays to functionally characterize human enhancers genome-wide
Our study provides a comprehensive framework for integrating different enhancer datasets and underscores the importance of accounting for assay-specific biases when interpreting enhancer activity. These findings refine enhancer identification using massively parallel reporter assays and improve the functional annotation of the human genome.
Authors
- Nathaniel D. Tippens (ORCID: https://orcid.org/0000-0003-4377-8691)
- Jin Liang (ORCID: https://orcid.org/0000-0001-6770-369X)
- Alden King-Yung Leung (ORCID: https://orcid.org/0000-0003-0559-2183)
- Timothy E. Reddy (ORCID: https://orcid.org/0000-0002-7629-061X)
- Keith Siklenka (ORCID: https://orcid.org/0000-0002-4663-6456)
- Anne E. Willis (ORCID: https://orcid.org/0000-0002-1470-8531)
- Xiuqi Pan
- John T. Lis (ORCID: https://orcid.org/0000-0003-1201-9406)
- Alejandro Barrera (ORCID: https://orcid.org/0000-0001-9244-9822)
- Haiyuan Yu (ORCID: https://orcid.org/0000-0001-7597-6049)
- Abdullah Ozer (ORCID: https://orcid.org/0000-0001-6861-2853)
- Zhou Zhou (ORCID: https://orcid.org/0000-0003-2856-7116)
- Yutong Zhu (ORCID: https://orcid.org/0000-0001-5276-7375)
- Li Yao (ORCID: https://orcid.org/0000-0002-7785-5563)
- J. Zhang (ORCID: https://orcid.org/0000-0002-2178-1307)
Institutions
- Duke University (US)
- Cornell University (US)
- Tri-Institutional PhD Program in Chemical Biology (US)
Publication Details
- Journal
- Genome biology
- Published
- 2025-11-03
- DOI
- https://doi.org/10.1186/s13059-025-03828-8
- Citations
- 3
- Primary Topic
- Genomics and Chromatin Dynamics
- Type
- article
- Field-Weighted Citation Impact
- 2.42
Funders
- National Institutes of Health