Duplex‐Specific DNase Signal Amplification Allows Attomolar Electrochemical Detection of MicroRNAs

Point-of-care biosensors for detecting blood-circulating nucleic acids hold significant potential for disease diagnosis. However, achieving both high sensitivity and accessibility remains a challenge. Electrochemical hybridization assays offer a promising approach for detecting specific nucleic acid sequences but are typically limited to picomolar concentrations. Here, a duplex-specific DNase-assisted electrochemical hybridization assay is presented, enabling highly sensitive nucleic acid detection. By incorporating duplex DNase, the method achieves detection limits as low as 1 attomolar, significantly enhancing sensitivity. This work paves the way for ultrasensitive nucleic acid detection in critical point-of-care applications.

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Publication Details

Journal
Small
Published
2025-11-02
DOI
https://doi.org/10.1002/smll.202507997
Primary Topic
Advanced biosensing and bioanalysis techniques
Type
article
Field-Weighted Citation Impact
0.00

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article

Duplex‐Specific DNase Signal Amplification Allows Attomolar Electrochemical Detection of MicroRNAs

Conor F. Hogan, Chamindie Punyadeera, George W. Greene, Dênio Emanuel Pires Souto et al.
Small
Advanced biosensing and bioanalysis techniques
article

Duplex‐Specific DNase Signal Amplification Allows Attomolar Electrochemical Detection of MicroRNAs

Conor F. Hogan, Chamindie Punyadeera, George W. Greene, Dênio Emanuel Pires Souto, Saimon Moraes Silva, Richard W. Tothill, Brian Abbey, Thiago Coimbra Pimenta, Vatsala Pithaih, Henry F. F. Bellette
article en

Abstract

Point-of-care biosensors for detecting blood-circulating nucleic acids hold significant potential for disease diagnosis. However, achieving both high sensitivity and accessibility remains a challenge. Electrochemical hybridization assays offer a promising approach for detecting specific nucleic acid sequences but are typically limited to picomolar concentrations. Here, a duplex-specific DNase-assisted electrochemical hybridization assay is presented, enabling highly sensitive nucleic acid detection. By incorporating duplex DNase, the method achieves detection limits as low as 1 attomolar, significantly enhancing sensitivity. This work paves the way for ultrasensitive nucleic acid detection in critical point-of-care applications.

SmallVol. 21(50)
Translational Research Institute (AU), Cancer Council Victoria (AU), La Trobe University (AU), Peter MacCallum Cancer Centre (AU), Victorian Comprehensive Cancer Centre (AU), Universidade Federal do Paraná (BR)
Department of Science and Technology, Ministry of Science and Technology, India, Garnett Passe and Rodney Williams Memorial Foundation, Tour de Cure, Medical Research Council, National Health and Medical Research Council
Openalex Percentile: Top 71%
Advanced biosensing and bioanalysis techniques
DOIOpen Access PDFOpenAlex
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