Electrochemical assessment of α-amylase inhibition by type 2 diabetes drugs: a cyclic voltammetry study
A new sensitive cyclic voltammetry method was developed for measuring the α-amylase inhibitory activity of six antidiabetic drugs commonly prescribed for type 2 diabetes: acarbose, amaryl, repaglinide, diamecron, dapagliflozin, and metformin. The electrochemical response of an enzyme-starch mixture was measured in the absence and presence of these drugs. Results indicated varying inhibitory potencies with IC50 values ranging from 6.770 to 41.423 µM, with acarbose as the most potent. UV-Vis absorption spectroscopy at 580 nm validated the cyclic voltammetry results, with very good concordance. This study highlights the utility of electrochemical assays for the rapid, quantitative screening of α-amylase inhibitors and supports their integration with spectroscopic methods for the evaluation of antidiabetic drugs.
Authors
- Elhafnaoui Lanez (ORCID: https://orcid.org/0000-0002-6543-2547)
- Aicha Adaika (ORCID: https://orcid.org/0000-0002-7829-709X)
- Touhami Lanez (ORCID: https://orcid.org/0000-0002-3978-7635)
Institutions
- Centre Hospitalo-Universitaire Bab El Oued (DZ)
- Life University (US)
Publication Details
- Journal
- Journal of Electrochemical Science and Engineering
- Published
- 2025-11-02
- DOI
- https://doi.org/10.5599/jese.2867
- Primary Topic
- Natural Antidiabetic Agents Studies
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Ministère de l'Education Nationale, de l'Enseignement Superieur et de la Recherche
- Direction Générale de la Recherche Scientifique et du Développement Technologique