Fasting and Refeeding Mediated Phospholipid Remodeling Plays an Important Role in Improving Meat Quality of Aged Laying Hens
ABSTRACT This study explored fasting‐induced physiological remodeling effects on meat quality in aged laying hens. Fasting for 15 days (F15) significantly reduced abdominal fat, intermuscular fat width, subcutaneous fat thickness, and liver index versus pre‐fasting (F0) ( p < 0.05), which recovered post‐refeeding. Breast/leg muscle quality was poorest at F15 but optimized by day 20 of refeeding (R20). Serum total cholesterol/triglycerides decreased during fasting ( p < 0.05), whereas low‐density lipoprotein rose, both normalizing post‐refeeding. Lipidomics identified 155 lipids (73.5% glycerophospholipids, mainly PC/PE) in pectoralis muscle; transcriptomics revealed 330 differentially expressed genes. Enriched pathways included glycerolipid/fatty acid metabolism. ACSL4 correlated with PC (14:0e/22:6), PC (14:0/20:4), and PC (14:0/18:2) (|R| > 0.7). Fasting upregulated ACSL4/LPCAT, enhancing acyl‐CoA and lipid reacylation to drive phospholipid remodeling, improving meat quality.
Authors
- Yadong Tian (ORCID: https://orcid.org/0000-0002-4215-5698)
- X. Q. Zhang (ORCID: https://orcid.org/0009-0005-2032-762X)
- Ruirui Jiang (ORCID: https://orcid.org/0000-0002-7863-6186)
- Jun Zhang (ORCID: https://orcid.org/0000-0003-1956-4087)
- Yanhua Zhang (ORCID: https://orcid.org/0000-0002-3135-5512)
- Donghua Li (ORCID: https://orcid.org/0000-0001-5721-0429)
- Lujie Zhang (ORCID: https://orcid.org/0000-0001-5669-6324)
- Yujie Gong (ORCID: https://orcid.org/0009-0004-6191-9990)
- Xiangtao Kang (ORCID: https://orcid.org/0000-0002-9628-7135)
Institutions
- Ministry of Agriculture (EE)
- Poultry Research Institute (CN)
- Henan Agricultural University (CN)
Publication Details
- Journal
- Animal Research and One Health
- Published
- 2025-10-31
- DOI
- https://doi.org/10.1002/aro2.70036
- Primary Topic
- Meat and Animal Product Quality
- Type
- article
- Field-Weighted Citation Impact
- 0.00