Comparison of three commercial DNA extraction kits and assemblers for AMR determinant detection in Pseudomonas aeruginosa and Enterobacter cloacae using long-read sequencing
This systematic comparison highlights trade-offs between DNA yield, integrity, and downstream assembly performance, demonstrating that assembler choice critically impacts the detection of AMR determinants. These findings provide practical guidance for optimizing long-read-based sequencing workflows to support AMR surveillance and genomic epidemiology.
Authors
- Basil Britto Xavier (ORCID: https://orcid.org/0000-0002-5897-0240)
- Bhanu Sinha (ORCID: https://orcid.org/0000-0003-1634-0010)
- Anurag Kumar Bari (ORCID: https://orcid.org/0000-0002-0693-0044)
- Tim T. Severs (ORCID: https://orcid.org/0009-0008-0015-6802)
- John W. A. Rossen (ORCID: https://orcid.org/0000-0002-7167-8623)
Institutions
- University Medical Center Groningen (NL)
Publication Details
- Journal
- Journal of Microbiological Methods
- Published
- 2025-10-31
- DOI
- https://doi.org/10.1016/j.mimet.2025.107317
- Citations
- 1
- Primary Topic
- Antibiotic Resistance in Bacteria
- Type
- article
- Field-Weighted Citation Impact
- 0.59
Funders
- European Commission
- HORIZON EUROPE Framework Programme