Tangeretin protects mice from sepsis-induced myocardial injury by inhibiting TRIM8 expression through upregulation of miR-20a-5p

In this study, tangeretin (TAN), a plant-derived flavonoid, was investigated for its role in sepsis-induced myocardial injury (SIMI).C57BL/6 mice were gavaged daily with 50 mg/kg TAN, while lentiviruses interfering with miR-20a-5p or TRIM8 expression were injected into the tail vein for three consecutive days.Then, a mouse model of sepsis was established by cecum ligation puncture (CLP).Levels of serum myocardial injury markers (CK-MB and cTnI) were detected by a fully automated analyzer.The histopathology of mouse myocardium was observed by HE staining.Apoptosis was detected by TUNEL staining in mouse myocardial tissues.TAN improved cardiac dysfunction and myocardial injury in CLP mice.TAN could upregulate miR-20a-5p and downregulate TRIM8 levels.The beneficial effects of TAN could be attenuated by downregulating miR-20a-5p or upregulating TRIM8.TAN protects mice from SIMI by inhibiting TRIM8 expression through upregulation of miR-20a-5p.