Dual transcriptomic profiling of <i>Staphylococcus aureus</i> endocarditis in a porcine model reveals strong parallels with human infection
To gain a full understanding of how bacteria infect tissues, it is necessary to characterize both the bacteria and the tissue at the time of infection. However, this analysis is very complex because it involves obtaining infected tissue directly from patients and analyzing it with minimal processing to preserve the characteristics of the natural state of infection. In this study, we examined the gene expression profiles of replaced heart valves from patients with <i>Staphylococcus aureus</i> infection and compared them with pig valves experimentally infected with the same bacteria. Our findings provide a detailed insight into the changes occurring in the infected tissue and the bacterial adaptations required for multiplication and survival on the valve tissue. Notably, the strong similarity observed between human and porcine valves confirms that the porcine endocarditis model closely mirrors the human condition, making it a valuable tool for testing new therapies against this serious infection.
Authors
- Rafael Sádaba (ORCID: https://orcid.org/0000-0003-3776-4744)
- Ana Navascués (ORCID: https://orcid.org/0000-0002-1372-9680)
- Carmen Ezpeleta
- Amaya Fernández‐Celis (ORCID: https://orcid.org/0000-0002-5627-7653)
- Íñigo Lasa (ORCID: https://orcid.org/0000-0002-6625-9221)
- David Gómez-Castillo (ORCID: https://orcid.org/0000-0001-7321-0453)
- Begoña García (ORCID: https://orcid.org/0000-0002-0476-4900)
- Virginia Álvarez (ORCID: https://orcid.org/0000-0002-9899-9259)
- Cristina Solano (ORCID: https://orcid.org/0000-0002-6207-1766)
- Carmen Gil (ORCID: https://orcid.org/0000-0002-8867-4442)
- Daniel Mouzo (ORCID: https://orcid.org/0000-0002-0383-5703)
- Natalia López‐Andrés (ORCID: https://orcid.org/0000-0003-3728-2606)
- Ivan Pasquier
- Aritza Conty
- Nahiara Garmendia-Antoñana
Institutions
- Complejo Hospitalario de Navarra (ES)
- Navarrabiomed (ES)
- King Abdullah University of Science and Technology (SA)
- Universidad de Navarra (ES)
Publication Details
- Journal
- mBio
- Published
- 2025-10-31
- DOI
- https://doi.org/10.1128/mbio.02316-25
- Primary Topic
- Infective Endocarditis Diagnosis and Management
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Ministerio de Ciencia, Innovación y Universidades
- European Commission
- Ministerio de Economía y Competitividad
- Gobierno de Navarra
- Instituto de Salud Carlos III
- European Regional Development Fund