A lab-on-a-chip system integrating DNA purification and loop-mediated isothermal amplification for the quantification of the toxic diatom <i>Pseudo-nitzschia multistriata</i>
A microfluidic system using DMA-based DNA purification for toxin-producing algae, combined with air-dried LAMP reagents stable at room temperature for up to five months, enables rapid and low-cost detection without the need for cold storage.
Authors
- Allison Schaap (ORCID: https://orcid.org/0000-0001-5391-0516)
- John Walk (ORCID: https://orcid.org/0009-0009-6760-4929)
- Daniel H. Rogers (ORCID: https://orcid.org/0009-0006-8340-874X)
- Reuben Forrester (ORCID: https://orcid.org/0000-0002-0016-7420)
- Christopher L. Cardwell (ORCID: https://orcid.org/0000-0003-1305-4174)
- Jonathan S. McQuillan (ORCID: https://orcid.org/0000-0001-9725-3378)
- Ahmed Alrefaey (ORCID: https://orcid.org/0009-0001-3191-3545)
- Julie Robidart (ORCID: https://orcid.org/0000-0001-9805-3570)
- Matthew C. Mowlem (ORCID: https://orcid.org/0000-0001-7613-6121)
- Fabrizio Siracusa
Institutions
- National Oceanography Centre (GB)
- University of Southampton (GB)
Publication Details
- Journal
- Sensors & Diagnostics
- Published
- 2025-10-31
- DOI
- https://doi.org/10.1039/d5sd00135h
- Primary Topic
- Marine Toxins and Detection Methods
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- UK Research and Innovation
- European Commission
- Stazione Zoologica Anton Dohrn
- National Oceanography Centre