A Review on Recent Advances in HPLC (High Performance Liquid Chromatography) Under Green Chromatography Methods

High-Performance Liquid Chromatography (HPLC) represents a fundamental analytical technique that has transformed chemical analysis across multiple sectors. Modern HPLC combines advanced column technologies (sub-2-μm particles, monolithic columns) with sophisticated detection systems and automated platforms, enabling superior separation efficiency and analytical precision. Major technological advances include the development of UHPLC, integration with mass spectrometry, and implementation of green chemistry principles, resulting in faster analyses and reduced environmental impact. The technology continues to evolve through AI integration, automated method development, and miniaturization trends, adapting to emerging analytical challenges in pharmaceuticals, environmental monitoring, and clinical diagnostics. Recent innovations focus on enhancing portability, reducing analysis times, and improving sustainability while maintaining analytical robustness and reliability.