Eco-Conscious UPLC Analysis for the Quantification of Adagrasib in Bulk and Tablets

Ultra Performance Liquid Chromatography (UPLC) is a new and simple method for figuring out how much Adagrasib is present in a pharmaceutical dose form. This approach is sensitive, quick, accurate, and repeatable. A Waters Alliance e2695 system (150 × 4.6 mm, 3.5 µm) with a Waters XBridge C18 column and a mobile phase of acetonitrile and formic acid (0.1% ) in a 70:30 v/v ratio was used to separate adagrasib. Detection was performed using a photodiode array detector at room temperature, with a flow rate (1.0 mL.min-1), and absorption measured at 269 nm. A Purity Flag of "No" in the Empower software, indicating that the purity angle is less than the purity threshold, confirmed that the Adagrasib peak is homogeneous. The method yielded a theoretical plate count greater than 2000 and a tailing factor not exceeding 1.0. The % RSD for peak area measurements was less than 1.0%. The process was verified in using ICH guidelines. Overall, the quantitative analysis of Adagrasib was carried out using a simple, cost-effective, suitable, accurate, precise, and reliable method.