A Novel Densitometric HPTLC Method Integrated with FTIR for The Detection of Rutin in Senna auriculata Flower Extract

The objective of this research was to create a reliable way to analyze flavonoids, mainly Rutin, in extract from the Senna auriculata flower with densitometric HPTLC and FTIR. Fresh flowers were extracted using methanol and analyzed on silica gel plates using toluene\:ethyl acetate\:formic acid as the mobile phase, looking for compounds with toluene (254 nm) and ethyl acetate (366 nm). Rutin was confirmed in the Standard Rutin and the extract, as the Rf values for both were 0.014, 0.017 for Standard Rutin and 0.032, 0.026 for the extract. There was 1.12% Rutin present in the raw material. The presence of phenolic, aromatic and ether functional groups in FTIR confirmed that the compounds are flavonoids. Using this approach, the process was reliable, effective, pollution-free and needed very little solvent. The method enables the generation of a radiation-proof, non-rinse herbal sheet mask for space missions, specifically those on the International Space Station (ISS).