Phytochemical and Antimicrobial Screening of Stem Bark of Thevetia Peruviana

This study examines the phytochemical and antimicrobial properties of methanolic extract of stem bark of Thevetia Peruviana. Methanol was used to extract the powdered stem bark that had been shade-dried, and once the solvent had been removed, the methanolic extract was put to column chromatography. α-amyrin, lupeol acetate, ursolic acid, thevefolin, and 3β, 6β, 19α-trihydroxyurs-12-en-28-oic acid (a new substance) were obtained through chemical analysis of the stem bark of this plant. Every single one of these compounds was characterized using spectral studies and physical attributes. The crude stem bark extract was tested for antibacterial and antifungal activity against selected bacteria and fungi by using the disc diffusion method. Significant antibacterial activity was demonstrated by the extract against E. coli (AI 0.87 at 1000 µg/disc and 0.72 at 500 µg/disc). Antifungal efficacy was demonstrated against A. niger (AI 0.63 at 1000 µg/disc and 0.45 at 500 µg/disc).