Agromyces endophyticus sp. nov., isolated from endosphere of garlic

An aerobic, Gram-stain-positive, rod-shaped bacterial strain, designated H17E-10^T, was isolated from the endosphere of garlic (<i>Allium sativum</i>) in Jeju, Korea. Growth was observed at 10-40 °C (optimum, 30 °C) and pH 7.0-10.0 (optimum, pH 8.0), with up to 6% (w/v) NaCl (optimum, 0%). blast analysis of 16S rRNA gene sequences revealed the highest sequence similarity of strain H17E-10^T with <i>Agromyces soli</i> MJ21^T (98.6%), followed by <i>Agromyces mediolanus</i> DSM 20152^T (98.5%) and <i>Agromyces indicus</i> NIO-1018^T (98.1%). According to phylogenetic analyses based on 16S rRNA gene sequences, strain H17E-10^T formed a cluster with <i>A. soli</i> MJ21^T, <i>A. mediolanus</i> DSM 20152^T and <i>A. indicus</i> NIO-1018^T. In contrast, the phylogenomic tree exhibited close relatedness between strain H17E-10^T and <i>Agromyces chromiiresistens</i> H3Y2-19a^T, which supported by the highest Orthologous Average Nucleotide Identity (OrthoANI; 87.7%) and digital DNA-DNA hybridization (32.7%) values with each other. The major menaquinone (MK) and polar lipids of strain H17E-10^T were MK-13 and diphosphatidylglycerol, phosphatidylglycerol and two unidentified glycolipids, respectively, with the presence of B-type peptidoglycan. The major fatty acids (>10%) of strain H17E-10^T were anteiso-C_{15 : 0}, iso-C_{16 : 0} and anteiso-C_{17 : 0}. The phenotypic, chemotaxonomic and genotypic data obtained in this study showed that strain H17E-10^T represents a novel species of the genus <i>Agromyces</i>, for which the name <i>Agromyces endophyticus</i> sp. nov. (type strain H17E-10^T=KACC 21836^T=NBRC 115478^T) is proposed.