Next-generation stress-inducible Komagataella phaffii promoter variants
Creating and characterizing variants of the P<sub>DH</sub> sequence supported the elucidation of the sequence-function relationships of this promoter. In addition, the surprisingly beneficial effects of a synthetic 10 bp sequence stretch opened up opportunities for further engineering of this system and extended the toolbox of efficient vector parts for methanol-free and growth-decoupled protein production with K. phaffii. Those additional promoter sequences will also support the construction of stable engineered strains with a balanced expression of multiple genes, as needed for e.g. multienzyme pathways and synthetic biology applications.
Authors
- Xavier Garcia‐Ortega (ORCID: https://orcid.org/0000-0001-7833-3655)
- Anton Glieder (ORCID: https://orcid.org/0000-0002-5986-9689)
- Christa Dörner
- Núria Bernat-Camps
- Katharina Ebner
- Francisco Valero (ORCID: https://orcid.org/0000-0003-0429-9620)
- Simona Scheipel
Institutions
- Beiersdorf (Germany) (DE)
- Universitat Autònoma de Barcelona (ES)
- Institute of Molecular Biotechnology (AT)
- Graz University of Technology (AT)
- Nawi Graz (AT)
- Austrian Centre of Industrial Biotechnology (Austria) (AT)
Publication Details
- Journal
- Microbial Cell Factories
- Published
- 2025-10-30
- DOI
- https://doi.org/10.1186/s12934-025-02825-7
- Citations
- 2
- Primary Topic
- Bacterial Genetics and Biotechnology
- Type
- article
- Field-Weighted Citation Impact
- 3.63
Funders
- TU Graz, Internationale Beziehungen und Mobilitätsprogramme
- Amt der NÖ Landesregierung
- Österreichische Forschungsförderungsgesellschaft
- Austrian Centre of Industrial Biotechnology
- Steirische Wirtschaftsförderungsgesellschaft
- Standortagentur Tirol
- Bundesministerium für Digitalisierung und Wirtschaftsstandort