Survival and genomic stability of Yersinia enterocolitica in environmental Acanthamoeba spp

All strains were internalized and survived up to 8 days in the collection strain and 16 days in the wild isolate. Intracellular persistence did not affect amoebal integrity or bacterial virulence profiles. Whole genome sequencing (WGS) revealed high genomic stability across strains, though specific mutations-such as in the <i>igaA</i> gene, involved in stress response-emerged after persistence in the collection strain. These findings suggest that <i>Acanthamoeba</i> spp. not only shields <i>Y. enterocolitica</i> from environmental stress but may also influence its genome and adaptive potential. This work expands the current understanding of <i>Y. enterocolitica</i> biology and highlights the role of FLA as reservoirs and potential drivers of bacterial evolution. Their contribution to the bacteria persistence and gene exchange warrants further investigation, particularly in the context of antimicrobial resistance and food safety.

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Publication Details

Journal
Frontiers in Cellular and Infection Microbiology
Published
2025-10-30
DOI
https://doi.org/10.3389/fcimb.2025.1642352
Primary Topic
Legionella and Acanthamoeba research
Type
article
Field-Weighted Citation Impact
0.00

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article

Survival and genomic stability of Yersinia enterocolitica in environmental Acanthamoeba spp

Rubén L. Rodríguez-Expósito, Elisabetta Delibato, María Reyes-Batllé, Stefano Morabito et al.
Frontiers in Cellular and Infection Microbiology
Legionella and Acanthamoeba research
article

Survival and genomic stability of Yersinia enterocolitica in environmental Acanthamoeba spp

Rubén L. Rodríguez-Expósito, Elisabetta Delibato, María Reyes-Batllé, Stefano Morabito, Margherita Montalbano Di Filippo, Valeria Michelacci, Valentina Libri, Paola Chiani, Eleonora Ventola, Jacob Lorenzo‐Morales, Manuela Marra, Isabel Fuentes, Samra Mannan
article en

Abstract

All strains were internalized and survived up to 8 days in the collection strain and 16 days in the wild isolate. Intracellular persistence did not affect amoebal integrity or bacterial virulence profiles. Whole genome sequencing (WGS) revealed high genomic stability across strains, though specific mutations-such as in the <i>igaA</i> gene, involved in stress response-emerged after persistence in the collection strain. These findings suggest that <i>Acanthamoeba</i> spp. not only shields <i>Y. enterocolitica</i> from environmental stress but may also influence its genome and adaptive potential. This work expands the current understanding of <i>Y. enterocolitica</i> biology and highlights the role of FLA as reservoirs and potential drivers of bacterial evolution. Their contribution to the bacteria persistence and gene exchange warrants further investigation, particularly in the context of antimicrobial resistance and food safety.

Frontiers in Cellular and Infection MicrobiologyVol. 15
Universidad de La Laguna (ES), Istituto Superiore di Sanità (IT), Instituto de Salud Carlos III (ES)
Istituto Superiore di Sanità, Instituto de Salud Carlos III
Openalex Percentile: Top 73%
Legionella and Acanthamoeba research
DOIOpen Access PDFOpenAlex
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