Alterations of Raman Profiles of Cystic Echinococcosis Serum After Treated With <scp>ABZ</scp> by <scp>SERS</scp>

Cystic echinococcosis (CE) is often treated with albendazole (ABZ). Surface-enhanced Raman scattering (SERS) has high sensitivity and specificity, as well as low sample consumption, but its ability to identify changes in serum compounds post-ABZ treatment is unclear. This study used SERS to characterize the Raman profiles of serum from 7 normal, 9 CE-model, and 10 ABZ-treated mice, analyzing profile differences. Tentative peak assignments revealed ABZ-induced biomolecular changes in protein structure (1000 cm^-1), uric acid (1130 cm^-1), amide III (1203 cm^-1), phospholipids, and amide I (1656 cm^-1). The top 10 principal components (PCs) accounted for 90% of the variance (PC1: 48.9%). Principal components analysis (PCA) loadings suggested these substances as markers of ABZ treatment. The results show that SERS combined with PCA is a rapid and effective method to observe serum changes in Echinococcus granulosus-infected subjects.