RAMAN FLUORESCENT THERAPEUTIC AND DIAGNOSTIC TECHNOLOGY AND THE RANGE OF ITS CLINICAL APPLICATION

The article presents the results of the development and clinical application of the domestic Raman fluorescence therapeutic and diagnostic technology (RFD), implemented on the basis of the hardware and software complex "InSpectr M". High analytical sensitivity and specificity of the method are shown in comparison with traditional fluorescence and microbiological diagnostic methods. The possibilities of using RFD in clinical microbiology, dentistry, as well as in the diagnosis of benign and malignant neoplasms of the oral organs and maxillofacial region are considered. Particular attention is paid to the digitalization of diagnostic and treatment processes in dental clinics, which meets the modern requirements of personalized and express medicine. The technique allows for express assessment of microbial association, mineralization of hard dental tissues, selection of antimicrobial drugs and monitoring of the effectiveness of treatment "on site". The work presents experimental and clinical data confirming the effectiveness and prospects of introducing RFD into medical practice. Objectives. To develop and implement a highly sensitive Raman fluorescence diagnostic and treatment technology for express diagnostics, monitoring and optimization of treatment of dental diseases in a polyclinic setting. Methodology. The study included the development of the hardware and software complex "InSpectr M", implementing RFD-technology using laser radiation at wavelengths of 405, 532 and 637 nm and the use of SERS substrates to increase sensitivity. The objects of the study were microbial suspensions (Pseudomonas aeruginosa, Morganella morganii, Staphylococcus aureus, etc.), hard tissues of teeth of patients with caries and fluorosis, as well as tissues of oral organs with neoplasms. Results. The developed RFD technology based on the InSpectr M complex is an innovative, highly sensitive and specific method of express diagnostics and treatment of dental diseases and pathologies of oral tissues. Its integration into the digital infrastructure of polyclinics contributes to a significant improvement in the quality and speed of medical care, and also opens up new opportunities for diagnosing oncological diseases.