Photo‐Induced Ultra‐Fast Duplex Invasion Targeting Long‐Range Double‐Stranded DNA Using Artificial Nucleotide

Genomic DNA stores genetic information and regulates functions of various biological processes. Genetic diseases can be caused by abnormal gene expression. Genetic manipulation requires sequence-selective recognition of double-stranded DNAs, various chemical approaches for double-duplex invasion have been developed. In the previous research, a photo-induced double-duplex invasion (pDDI) is introduced that uses artificial nucleotides, 3-cyanovinylcarbazole nucleoside (^CNVK) as photo-crosslinker and 5-cyanouridine (^CU) as inter-probe photo-crosslinking inhibitor. In this study, while investigating the invasion mechanism of the pDDI, the invasion independence of pDDI probes is discovered and proposed a photo-induced duplex invasion (pDI) that achieves fast invasion using only ^CNVK while still maintaining a high invasion efficiency. This rapid pDI approach provides a powerful new tool for site-specific manipulation of genomic DNA.