SDS PAGE Analysis Of Protein In Skeletal Muscles of Labeo rohita During Immunomodulation And Aeromoniasis

The present study investigates the alterations in protein profiles of skeletal muscle in Labeo rohita during immnomodulation and aeromoniasis. Nine months old fish (4 groups) were used in the present investigation. Groups A and B were treated with Aqua Fix for 4 days, on day 5 fish of group B and C were infected with Aeromonas hydrophila @ 10-6CFU/fish(fish of group C were fed with normal diet for 4 days).Controls (group D) were untreated and uninfected. Three fish from group A were necropsied on day 2 and 5 after Aqua Fix treatment (6, and 9 of experimental period) and 3 fish from group B and C on day 1, and 4 after infection (6, and 9 of experimental period). Three fish from controls (group D) were also necropsied on the same designated days for comparison. Qualitative muscle protein profile by SDS-PAGE was analysed. The electrophoretic profiles showed distinguishable qualitative changes in protein bands across groups. Group C (Infected fish) showed degradation of muscle protein due to the stress caused by the pathogen and tissue damage. Group A (immunostimulated fish) showed up gradation of protein expression suggesting enhanced metabolic and immune response. Group B (fish which received aqua fix and infection) showed loss of 5 protein bands and synthesis of 2 new bands which indicates the partial restoration of protein profiles and protective effect of Aqua fix .This study supports the role of immunostimulants in disease resistance in Aquaculture and use of SDS-PAGE to monitor physiological changes at the proteomic level.