Efficacy of ‘co–ins’–sync protocol on fertility of anestrus buffaloes

Major production factor influencing buffaloes is their reproductive efficiency. Out of the various reproductive disorders observed in buffaloes, postpartum anestrum is the principal cause of economical loss to the farmers. The present study was designed to assess the effect of CO–sync and ‘CO–INS’–sync protocol with fixed time insemination in post-partum anestrus buffaloes which had failed to conceive after progesterone therapy with CIDR. Apparently healthy graded true anestrous parous Murrah buffaloes (n=12) with 5–10 years of age which failed to get conceived after CIDR treatment (75–120 days) were selected and divided into two groups (n=6), Group I is Cosync protocol and Group II, is addition of insulin and metformin in Cosync and is termed as Co–ins–Sync protocol. All the buffaloes were examined by ultrasonography for the ovulatory status and fixed time breeding was performed. Plasma insulin and Thyroid Stimulating Hormone (TSH) was studied during day 0 and 9 of the treatment. In Cosync group one out of six buffaloes (16.6%) became pregnant, while two out of six (33.3%) buffaloes were pregnant in Co–ins–sync group. Significant increase in insulin and decrease in TSH values was observed on comparison with day 0 and 9 of the treatment in Co–ins–Sync group and attained the physiological reference range. From the present study, it could be concluded that insulin along with Cosync protocol ie., CO–INS’–SYNC would be a successful approach in postpartum anestrum buffaloes failed to conceive even after progesterone protocol.