LIM kinase/inhibitor binding study in cell lysates using microscale thermophoresis in the red spectrum
This work establishes the first detailed production, characterization, and use of miRFP670 in MST-based evaluation of biomolecular interactions under near-native conditions. miRFP670 operates in a spectral region (red) with minimal cellular autofluorescence, enhancing signal specificity and sensitivity. This approach offers a straightforward and broadly applicable, purification-free platform for early-stage drug discovery.
Authors
- Reine Nehmé (ORCID: https://orcid.org/0000-0002-5288-7702)
- Muriel Sebban (ORCID: https://orcid.org/0000-0002-7907-1092)
- Fabienne Godin
- Solweig Chartier
- Gaël Coadou (ORCID: https://orcid.org/0000-0003-0979-6706)
- Rouba Nasreddine (ORCID: https://orcid.org/0000-0003-1382-4036)
- Elodie Villalonga (ORCID: https://orcid.org/0009-0004-2042-0421)
- Béatrice Vallée (ORCID: https://orcid.org/0000-0002-5161-354X)
- Bérengère Claude (ORCID: https://orcid.org/0000-0003-4951-1437)
- Alexandra Launay
- Mélanie RAPETO
Institutions
- Université d'Orléans (FR)
- Centre National de la Recherche Scientifique (FR)
- Laboratoire de Chimie Moléculaire (FR)
- Normandie Université (FR)
- Centre de Biophysique Moléculaire (FR)
- Institut de Chimie Organique et Analytique (FR)
- Laboratoire COBRA (FR)
- Université de Rouen Normandie (FR)
Publication Details
- Journal
- Analytica Chimica Acta
- Published
- 2025-10-29
- DOI
- https://doi.org/10.1016/j.aca.2025.344837
- Citations
- 1
- Primary Topic
- Field-Flow Fractionation Techniques
- Type
- article
- Field-Weighted Citation Impact
- 1.33