Cerebrospinal fluid extracellular vesicle-derived miR-9-3p in spinal cord injury with neuroprotective implications and biomarker development

Spinal cord injury (SCI) often results in severe disability, and early detection of molecular changes is crucial for guiding treatment. In both rat and human samples, we observed a significant increase in cerebrospinal fluid (CSF)-derived extracellular vesicle (EV) miR-9-3p after SCI, prompting further investigation into its role. In a rat model, miR-9-3p levels were significantly lower at the injured spinal levels but higher in the motor cortex, where astrocytes showed the highest expression. Functional analyses revealed that miR-9-3p regulates energy metabolism, immune activity, and oxidative stress in neurons, inducing transcriptional changes suggestive of stress adaptation and synaptic remodeling. These findings demonstrate that EV-associated miR-9-3p modulates injury responses by reducing energy demands and supporting structural and functional adaptation, establishing it as a promising biomarker and therapeutic target for acute SCI.

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Publication Details

Journal
Communications Biology
Published
2025-10-27
DOI
https://doi.org/10.1038/s42003-025-08947-3
Citations
1
Primary Topic
Extracellular vesicles in disease
Type
article
Field-Weighted Citation Impact
0.81

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article

Cerebrospinal fluid extracellular vesicle-derived miR-9-3p in spinal cord injury with neuroprotective implications and biomarker development

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1 citations
Communications Biology
Extracellular vesicles in disease
0.81
article

Cerebrospinal fluid extracellular vesicle-derived miR-9-3p in spinal cord injury with neuroprotective implications and biomarker development

Takeshi Maeda, Kaori Yasutake, Narihito Nagoshi, Chris Kato, Masaya Nakamura, Hideyuki Okano, Kota Suda, Satoru Morimoto, Munehisa Shinozaki, Keitaro Ito, Yoshiyuki Yato, T. Tanaka
article en
1 citations

Abstract

Spinal cord injury (SCI) often results in severe disability, and early detection of molecular changes is crucial for guiding treatment. In both rat and human samples, we observed a significant increase in cerebrospinal fluid (CSF)-derived extracellular vesicle (EV) miR-9-3p after SCI, prompting further investigation into its role. In a rat model, miR-9-3p levels were significantly lower at the injured spinal levels but higher in the motor cortex, where astrocytes showed the highest expression. Functional analyses revealed that miR-9-3p regulates energy metabolism, immune activity, and oxidative stress in neurons, inducing transcriptional changes suggestive of stress adaptation and synaptic remodeling. These findings demonstrate that EV-associated miR-9-3p modulates injury responses by reducing energy demands and supporting structural and functional adaptation, establishing it as a promising biomarker and therapeutic target for acute SCI.

Communications BiologyVol. 8(1)
Keio University (JP), Spinal Injuries Center (JP), Murayama Medical Center (JP), National Hospital Organization (JP)
Japan Agency for Medical Research and Development, Nippon Zoki Pharmaceutical, Kaken Pharmaceutical, Keio University, Daiichi Sankyo Foundation of Life Science, National Center of Neurology and Psychiatry, Chugai Pharmaceutical, Japan Society for the Promotion of Science, Daiichi Sankyo Europe
Openalex Percentile: Top 22%
Extracellular vesicles in disease
0.81
DOIOpen Access PDFOpenAlex
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