Nitrate Monitoring in Semi-Urban Groundwater of Northeastern Saudi Arabia

Monitoring nitrate levels in water is critical to protect public health and ensure compliance with regulatory standards. This study provides a comprehensive evaluation of four analytical techniques—test strips, ion-selective electrodes (ISE), colorimetric methods, and titration—to assess nitrate levels in a variety of water sources, including standard solutions, rainwater, bottled water, and groundwater from both shallow and deep wells located in semi-urban regions of Saudi Arabia. Each method was assessed for sensitivity, accuracy, detection limits, reproducibility, and operational practicality. Test strips offer rapid, low-cost screening but consistently underestimate nitrate concentrations, particularly at low levels. The ISE demonstrated broad applicability and reliable performance across a wide concentration range when properly calibrated, making it suitable for both field and laboratory applications. Colorimetric methods provide excellent sensitivity for trace-level detection, whereas titration delivers the highest accuracy for high-nitrate samples despite its time-intensive nature. By calibrating and validating the methods against certified standards, we quantitatively demonstrated their reliability through statistical measures such as precision and accuracy rates. Moreover, the application of Geographic Information System (GIS) techniques in spatial analysis has revealed significant differences in the distribution of nitrates. Notably, shallow wells located in the northern regions surpass the 50 mg/L limit set by the World Health Organization (WHO), thereby indicating the presence of localized contamination hotspots. This study is among the first to systematically compare nitrate detection methods across a wide range of water types in a semi-urban area of Saudi Arabia. Building on a detailed analysis of each method, we underline the crucial need for the strategic selection of nitrate analysis techniques. This selection should be tailored to specific operational contexts, accuracy requirements, and concentration ranges to guide stakeholders towards more informed decision-making. These findings provide actionable guidance for public health officials and water managers to prioritize monitoring, safeguard drinking-water sources, and mitigate nitrate-related health risks in semi-urban communities.