Comprehensive real-time metabolic profiling of peripheral blood mononuclear cells reveals important methodological considerations for immunometabolism research
This study demonstrates that key experimental variables including blood processing time and isolation method critically affect the reliability and biological relevance of PBMC metabolic assessments, highlighting the importance of protocol standardisation for accurate bioenergetic biomarker measurements.
Authors
- Alex J. Wadley (ORCID: https://orcid.org/0000-0002-1820-8446)
- Megan K. L. MacLeod (ORCID: https://orcid.org/0000-0003-1843-8580)
- Fendi Pradana
- Jonathan Barlow
Institutions
- Tadulako University (ID)
- Arthritis UK (GB)
- University of Birmingham (GB)
Publication Details
- Journal
- Frontiers in Immunology
- Published
- 2025-10-29
- DOI
- https://doi.org/10.3389/fimmu.2025.1676550
- Citations
- 1
- Primary Topic
- Metabolomics and Mass Spectrometry Studies
- Type
- article
- Field-Weighted Citation Impact
- 0.81
Funders
- Society for Endocrinology
- Medical Research Council