GC/MS Analysis, Cytotoxicity, and Antimicrobial Properties of Six Moroccan Essential Oils Traditionally Used for COVID-19 Prevention

The COVID-19 pandemic has reignited interest in traditional medicinal plants as potential therapeutic agents. This study examined the chemical composition, cytotoxicity, and antimicrobial activity of essential oils from six Moroccan medicinal plants, namely, <i>Eucalyptus globulus</i>, <i>Artemisia absinthium</i>, <i>Syzygium aromaticum</i>, <i>Thymus vulgaris</i>, <i>Artemisia alba</i>, and <i>Santolina chamaecyparissus</i>, which are commonly used by the Moroccan population for COVID-19 prevention. The chemical composition of each essential oil was determined using gas chromatography-mass spectrometry (GC-MS) to identify key compounds. Cytotoxicity was evaluated in the Vero E6 cell line, which is frequently used in SARS-CoV-2 research, using the neutral red assay, with oil concentrations ranging from 25 to 100 µg/mL. Antimicrobial activity was tested against standard reference strains, including <i>Escherichia coli</i> (ATCC 25922), <i>Pseudomonas aeruginosa</i> (ATCC 27853), <i>Staphylococcus aureus</i> (ATCC 25923), <i>Candida albicans</i> (ATCC 10231), and <i>Bacillus subtilis</i> (ATCC 6633), using the disc diffusion method. GC-MS analysis revealed significant components such as spathulenol (15%) and caryophyllene oxide (7.67%) in <i>Eucalyptus globulus</i> and eugenol (54.96%) in <i>Syzygium aromaticum</i>. Cytotoxicity assays indicated that higher concentrations of essential oils significantly reduced cell viability, with <i>Thymus vulgaris</i> showing the highest IC₅₀ (8.324 µM) and <i>Artemisia absinthium</i> the lowest (18.49 µM). In terms of antimicrobial activity, <i>Eucalyptus globulus</i> had the strongest effect, with a 20 ± 0.00 mm inhibition zone against <i>Bacillus subtilis</i>, whereas both <i>Syzygium aromaticum</i> and <i>Artemisia herba-alba</i> had a 12.25 ± 0.1 mm inhibition zone against the same strain. These findings suggest that these essential oils have significant therapeutic potential, particularly in combating antimicrobial resistance and exerting cytotoxic effects on viral cell lines. Further research is necessary to explore their mechanisms of action and ensure their safety for therapeutic use.