Analytical Validation of a Second-Generation Methotrexate Immunoassay
The assay performed well based on the validation experiments and is suitable for most clinical applications involving methotrexate. However, the significant interference with DAMPA highlights the necessity for careful assay selection and interpretation of glucarpidase-treated patients.
Authors
- J.C.P. Garrett
- Andrew N. Hoofnagle (ORCID: https://orcid.org/0000-0002-6449-0243)
- Diane Yamaguchi
- Michael Schmeling
- Rebecca Wilson (ORCID: https://orcid.org/0000-0003-4064-5420)
- Erin Kuest
Institutions
- Cincinnati Children's Hospital Medical Center (US)
- University of Washington (US)
- University of Cincinnati Medical Center (US)
Publication Details
- Journal
- The Journal of Applied Laboratory Medicine
- Published
- 2025-10-02
- DOI
- https://doi.org/10.1093/jalm/jfaf166
- Primary Topic
- Acute Lymphoblastic Leukemia research
- Type
- article
- Field-Weighted Citation Impact
- 0.00