Development of GALDI-FT-ICR-MS Methods for the Analysis of Xylan Oligomers

Xylans, a highly abundant type of hemicelluloses in plant-based biomass, are currently being investigated as a renewable substitute for fossil resources. Thus, understanding their properties and structure is crucial for efficient utilization. This study aims to develop analytical methods for characterizing the soluble and insoluble compounds of xylans, using graphite-assisted laser desorption/ionization combined with Fourier-transform ion cyclotron resonance mass spectrometry (GALDI-FT-ICR-MS). A beechwood xylan was utilized as a representative sample for this work. Infrared (IR) and nuclear magnetic resonance (NMR) spectroscopy were additionally used to obtain further structural information. The results from the MS analyses indicate that liquid- and solid-state GALDI-MS techniques can aid in the characterization of the xylan molecules, and both ionization modes were found to be essential for a comprehensive understanding of xylans. The findings of this study may contribute to a deeper understanding of the structural characteristics of xylans and assist in the efficient application of these polysaccharides.