Identification of Auxin, Cytokinin, Transcription Factors, and Other Zygotic Embryogenesis-Related Genes in Persea americana: A Transcriptomic-Based Study

Zygotic embryogenesis is a key process in the development and propagation of avocado (<i>Persea americana</i>). Plant growth regulators, particularly auxins and cytokinins, play a crucial role in regulating this process. In this study, a transcriptomic analysis was performed to identify and characterize the expression of genes related to biosynthesis, transport, signaling, or response to auxins and cytokinins during different stages of embryonic development, as defined by the varying sizes of collected fruits. Additionally, several transcription factors and genes related to embryogenesis were analyzed. The results reveal dynamic patterns of gene expression that suggest a coordinated interaction between these PGRs in embryo formation and differentiation. This study provides key insights into the molecular mechanisms that regulate avocado zygotic embryogenesis, with potential applications in biotechnology and plant propagation.