Two proteins, one goal: ELISAs based on p32 and L1R for LSDV antibodies detection
Both assays demonstrated high immunoreactivity and strong concordance with the VNT. These results support the suitability of both antigens for use in serological assays and suggest that a combined, multi-target ELISA approach could enhance diagnostic sensitivity. Once validated for routine use, these novel tools may significantly improve large-scale, cost-effective serological surveillance of LSDV in endemic and at-risk regions.
Authors
- Stefano Baselli (ORCID: https://orcid.org/0000-0002-7032-7442)
- Giulia Pezzoni (ORCID: https://orcid.org/0000-0002-6718-5658)
- Milovan Milovanović (ORCID: https://orcid.org/0000-0002-5277-8514)
- Anna Castelli (ORCID: https://orcid.org/0000-0002-4451-7330)
- Arianna Bregoli
- Bernd Hoffmann (ORCID: https://orcid.org/0000-0001-5358-6445)
- Manuel Corsa
- Benedetta Zanetti
Institutions
- Friedrich-Loeffler-Institut (DE)
- Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna "Bruno Ubertini" (IT)
Publication Details
- Journal
- Frontiers in Veterinary Science
- Published
- 2025-10-28
- DOI
- https://doi.org/10.3389/fvets.2025.1695369
- Primary Topic
- Poxvirus research and outbreaks
- Type
- article
- Field-Weighted Citation Impact
- 0.00