Novel Model for Stomatal Conductance: Enhanced Accuracy Under Variable Irradiance and CO2 in C3 Plant Species

This study analyzes stomatal conductance (<i>g</i>_sc) in <i>Trifolium repens</i> L., <i>Lolium perenne</i> L., and <i>Triticum aestivum</i> L. under varying environmental conditions. Light-response curves for photosynthesis (<i>A</i>_n-<i>I</i>) at 420 μmol mol^-1 CO₂ were used to determine saturating irradiance (<i>I</i>_sat) using a light-response model for photosynthesis, and CO₂-response curves for photosynthesis (<i>A</i>_n-<i>C</i>_i) were measured at <i>I</i>_sat and half <i>I</i>_sat for these C₃ plant species. The Ball-Woodrow-Berry (BWB) model, Medlyn model, and a new model were compared for their ability to describe the net photosynthetic rate (<i>A</i>_n) relative to <i>g</i>_sc under changing irradiance or CO₂. The BWB model overestimated <i>g</i>_sc response, simplifying stomatal behavior, while the Medlyn model deviated at high <i>A</i>_n values, indicating limitations in dynamic responses. The new model showed a better empirical fit under the tested conditions, achieving high <i>R</i>² values and low AIC values across all three species, and demonstrated a strong alignment with empirical data. Our findings highlight the complexity of <i>g</i>_sc regulation and the need for improved models to better represent stomatal dynamics under different environmental conditions. This research is vital for optimizing water use efficiency, enhancing crop productivity, and understanding plant resilience to climate change.