Probing the interaction of Iron Oxide Nanoparticles (IONPs) with dsDNA in presence of UV–Vis light

Present investigation has focused on the use of electrochemical tool to probe the interaction between the double-stranded DNA (dsDNA) and iron oxide nanoparticles (IONPs). IONPs are synthesized and characterized for their size and functional groups. Electrochemical scans are recorded while the dsDNA and the IONPs are dispersed in solution and undergone ultraviolet–visible (UV–Vis) light exposure from Xe lamp, which generated only a marginal current signal from the adenine and guanine nucleotides. The electrodes are thereafter modified with dsDNA and IONPs while dipped inside the phosphate buffer (PB), the exposure of Xe lamp generated electrochemical signals from adenine and guanine with peaks at 1.2 V and at 0.9 V, indicative of their release from the dsDNA strands. Repairable single strand breakage of dsDNA by UV radiation is indicated in presence of IONPs. Groove binding is the major mechanism of interaction between dsDNA and IONPs. The fast repair of the dsDNA strands has been supported from the electrochemical and also spectroscopic measurements indicated only the single strand damages of dsDNA in presence of IONPs due to UV–Vis light exposures.