Assessment of Wound Healing activity of Polyherbal Gel, by “Burn Wound Model” in Wistar rats

Aim: Burns are significant worldwide healthcare concern, often difficulty by the existence of microorganism like Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli in the wounds. Burn damages first line of defence against microorganism. Number of allopathic formulations are commonly used for the treatment of burn wound, but they cause skin irritation and delay wound healing. The aim of the present study was comparative assessment of polyherbal gel preparations for cutaneous application of healing agents at the time of burn wound to quickly skin heal. Method: In the present study, polyherbal gel formulations containing 10% hydroalcoholic (Ethanol/Methanol and Water) extract of Calendula officenalis, Turmeric, Azairachta indica and Aloe-vera at different concentration are prepared. The extraction of selected plants done by hot extraction and cold maceration methods. Gel was topically applied on wistar rats, weight between 150 gm.to 200 gm. of both sexes by using burn wound model. 5% betadine ointment are used as marketed standard preparation. The effectiveness and safety of polyherbal formulation was evaluated. Result: The result showed all the optimize gel formulations exhibited significant wound healing (P<0.0001) response. Differences in burn wound healing were observed between varied formulated gel formulations (F-9 = 84.68±3.12%, F-6 = 79.93±2.26% and F-2 = 75.63±2.96% respectively) compared with negative control (65.84±2.26%) and standard treated (86.51±2.89%) animals’ groups. Conclusion: The data obtained from the experiential study were encouraging and the data exhibited the hydroalcoholic (ethanol and water) extract significantly good wound healing response (F-6 and F-9) compared to methanol and water extract (F-2) and negative control. The wound contraction rate and epithelization rate (P<0.0001.) is excellence in hydroalcoholic preparation F-9: 25.83±1.47 and F-6: 29.5±1.87 days as compared to methanolic extract formulation F-2: 33.67±1.21 and negative controlled treated (35.67±1.63 days) animals’ groups. The result indicates that the prepared polyherbal gel formulation has accelerated the burn wound healing process and effective in skin injuries and burn.