Ultrastructural characterization of extracellular vesicles produced by <i>Encephalitozoon cuniculi</i> -infected B-1 cells
Herein, we analyzed the ultrastructure of the EVs released by <i>E. cuniculi</i>-infected B-1 cells or uninfected. Clusters of exosomes, also called intraluminal vesicles (ILVs), were surrounded by a membrane that was continuous with the plasma membrane of B-1 cells, characterizing the EVs known as multivesicular cargo (MVC). Migrasomes corresponded to clusters of exosomes located in the projection of the cell membrane. Ectosomes originated via the budding of the plasma membrane. To conclude, we showed that <i>E. cuniculi</i>-infected B-1 cells or B-1 CDP secrete large amounts of EVS especially after challenge with <i>E. cuniculi</i>.
Authors
- Elizabeth Cristina Pérez (ORCID: https://orcid.org/0000-0003-3038-9902)
- Maria Anete Lallo (ORCID: https://orcid.org/0000-0003-3578-3087)
- Anuska Marcelino Alvares-Saraiva (ORCID: https://orcid.org/0000-0001-8899-4182)
- Uziel Castillo-Velázquez (ORCID: https://orcid.org/0000-0002-0342-1703)
- Patrícia Xander (ORCID: https://orcid.org/0000-0002-6329-6449)
- Diva Denelle Spadacci-Morena (ORCID: https://orcid.org/0000-0003-1225-1715)
- Alicia Herrera Gutiérrez
- Rayane Cristina Bego
Institutions
- Universidad Autónoma de Nuevo León (MX)
- Instituto Butantan (BR)
- Universidade Paulista (BR)
- Universidade Federal de São Paulo (BR)
Publication Details
- Journal
- Ultrastructural Pathology
- Published
- 2025-10-28
- DOI
- https://doi.org/10.1080/01913123.2025.2580509
- Primary Topic
- Amoebic Infections and Treatments
- Type
- article
- Field-Weighted Citation Impact
- 0.00