Challenges, innovations and considerations for the use of tongue swabs in Mycobacterium tuberculosis complex detection
MTBC detection from serially collected TS was variable, but Ct values were consistent across swab order and storage conditions. VBB improved yield on qPCR, and Ultra detection was enhanced with diluted SR buffer, highlighting the value of optimized processing. These findings support the continued development of TS-based TB diagnostics.
Authors
- Lesley Scott (ORCID: https://orcid.org/0000-0001-6573-9525)
- Keneilwe Peloakgosi-Shikwambani
- Anura David (ORCID: https://orcid.org/0000-0003-1590-198X)
- Wendy Stevens (ORCID: https://orcid.org/0000-0003-0237-165X)
- Zanele Nsingwane
- Violet Molepo
Institutions
- National Health Laboratory Service (ZA)
- University of the Witwatersrand (ZA)
Publication Details
- Journal
- PLoS ONE
- Published
- 2025-10-27
- DOI
- https://doi.org/10.1371/journal.pone.0325585
- Primary Topic
- Mycobacterium research and diagnosis
- Type
- article
- Field-Weighted Citation Impact
- 0.00