ASSESSMENT OF BIOCHEMICAL MARKERS OF OXIDATIVE STRESS IN WOMEN WITH HYPERTENSIVE DISORDERS OF PREGNANCY

changes in the epidermis were examined with hematoxylin and eosin staining, and melanosomes were visualized using the Fontan-Mason staining method.The preparations were examined using a Carl Zeiss Primo Star microscope (Germany) equipped with a Zeiss AxioCam ER 5s digital camera (Germany) and ZEN 2 (blue edition) image capture software (Germany).The morphometric determination of the epidermal thickness, % of the epidermal area occupied by melanosomes was performed using the freely distributed ImageJ software, and then the synthetic index of "opacity index" was mathematically determined -the product of the average epidermal thickness by % of the area occupied by melanosomes.Statistical analysis was performed.Results.The studied parameters in the control group were as follows: epidermal thickness 32,635,39 mkm.The square of the epidermis section occupied by MS was 0,770,24%.Thus, the opacity index for the epidermis of the control group was 0,251 units.This value was also taken as 100%.On day 31, the experimental animals of both groups received the same UV exposure.The thickness of the epidermis reached 54,8910,58 mkm, the % of the epidermal area occupied by melanosomes was 1,210,32%, so the opacity index = 54,89 1,21% = 0,664 units, or 264,3%.For the group of free readaptation, the thickness of the epidermis on the 45th, 61st and 121st days of the experiment was 40,84,8 mkm, 49,9311,16 mkm, 45,189,44 mkm, respectively.% of MS area reached 1,030,16%, 0,910,13%, 0,910,25% and opacity index, respectively, 0,42 units, or 167,2%, 0,454 units, or 180,8%, 0,411 units, or 163,6%.For the hydroquinone depigmentation group, the thickness of the epidermis on the 45th, 61st and 121st days of the experiment reached 33,054,81 mkm, 43,6813,08 mkm, 53,495,65 mkm, respectively.The % of MS area was 0,920,27%, 0,790,2%, 0,750,2% and the opacity index was 0,304 units, or 121,0%, 0,345 units, or 137,3% and 0,401 units, or 159,7%, respectively.Conclusions.UV irradiation leads to a reliable increase in epidermal thickness, melanosome density in the epidermis on day 31 and then gradually decreases at different rates.The opacity index in both groups was markedly increased during all stages of observation.The density of MS has no significant differences between the experimental groups, while the opacity index on days 45 and 61 of the experiment was reliably lower in the hydroquinone depigmentation group.